mitochondrion targeted antioxidant mito tempo  (MedChemExpress)

Journal: Hepatology Communications

Article Title: Antioxidant interventions reduced cytokine-induced pyroptosis of peripheral MAIT cells in patients with HBV-related cirrhosis

doi: 10.1097/HC9.0000000000000714

Figure Lengend Snippet: Antioxidants reduced the cytokine-induced pyroptosis of mucosal-associated invariant T (MAIT) cells. (A–G) Peripheral blood mononuclear cells from healthy controls, patients with compensated liver cirrhosis, and patients with decompensated liver cirrhosis were treated with IL-18 or IL-12 stimulants with polyphenols, MTA, or iACAT for 24 hours, respectively. The levels of DCFH-DA MFI, FLICA caspase-1, IFN-γ, TNF-a, GZMB, CD107a, and IL-17A expressed in the MAIT cells were determined by flow cytometry. (A–G) Wilcoxon signed-rank test. * p <0.05, *** p <0.001, and **** p <0.0001, ns, non-signifcant. Abbreviations: MFI, mean fluorescence intensity; polyphenols, resveratrol and oleuropein; MTA, Mito-TEMPO and MitoQ; iACAT, Avasimibe and K-604.

Article Snippet: For the intervention with antioxidants, the treatments included the following: (1) medium only, (2) the addition of IL-12 or IL-18 at a concentration of 200 ng/mL, and (3) the same combination supplemented separately with polyphenols (ie, resveratrol [5 μM, APExBIO] and oleuropein [0.25 μM, APExBIO]); iACAT (ie, avasimibe [0.25 μM, Selleck], and K-604 [0.05 μM, MCE]; and mitochondria-targeted antioxidants (MTA) (ie, Mito-TEMPO [5 μM, Merk] and MitoQ [0.05 μM, MCE]).

Techniques: Flow Cytometry, Fluorescence